Biology RP 1Combined + SeparateTrilogy RP 1
Use a light microscope to observe plant and animal cells
Aim: Prepare, observe, draw and label plant and animal cells using a light microscope, including a magnification scale.
Variables
- Independent
- type of prepared cell specimen or magnification being compared
- Dependent
- structures resolved and measurements made in the microscope image
- Control
- specimen thickness and preparation method
- stain identity and amount
- lighting and focus procedure
- calibrated scale or the same image-measurement method
Method
- Place a thin specimen on a clean slide; add a suitable stain if instructed, then lower a coverslip at an angle to reduce trapped air bubbles.
- Put the slide on the stage and begin with the lowest-power objective lens.
- Use the coarse focus to find the specimen, then sharpen it with fine focus; use fine focus only at high power.
- Centre the specimen before changing to a higher-power objective and adjust the light if needed.
- Draw several representative cells using clear single lines, add labels and include a scale or calculated magnification.
- Repeat with the other specimen type and compare visible structures.
Risks and safety
- Wear eye protection when using stains and avoid skin contact; follow the school's reagent guidance.
- Handle glass slides and coverslips by their edges and report chips or breakages.
- Carry the microscope with two hands and keep it away from the bench edge.
Typical results and conclusion
Label only structures supported by the image. Calculate total magnification from the eyepiece and objective, or use an image scale to estimate real size, stating units and sensible precision.
Calculation: Total magnification = eyepiece magnification × objective magnification. Use a scale bar to estimate real size.
Common exam mistake
Drawing artistic shading or labelling structures that the image does not actually resolve.
Revise it in the Cell Biology notes →Full Biology practicals guide →
Biology RP 2Separate Biology only
Separate Biology: investigate antibiotics or antiseptics
Aim: Use aseptic technique to compare how antibiotics or antiseptics affect bacterial growth on agar by measuring clear zones of inhibition.
Variables
- Independent
- identity or concentration of the antibiotic or antiseptic on each disc
- Dependent
- mean diameter or calculated area of the clear zone around the disc
- Control
- bacterial strain and starting culture
- agar depth and plate size
- disc size and treatment volume
- incubation temperature and time
- solvent-only control disc
Method
- Disinfect the bench, wash hands and label the base of a sterile agar plate before inoculation.
- Sterilise the inoculating loop in a flame as instructed, let it cool, then use it to spread the bacterial culture evenly across the agar.
- Open the lid only slightly and briefly; place sterile treatment discs on the agar with sterile forceps.
- Include a control disc carrying only the solvent, then replace the lid promptly.
- Tape the lid in a few places without sealing all the way around, invert the plate and incubate at no more than 25 °C.
- Keep the incubated plate closed; measure two perpendicular clear-zone diameters and calculate a mean, or calculate area with πr².
- Repeat each treatment on separate plates and compare mean clear-zone sizes.
Risks and safety
- Use only the school-approved microorganism and follow trained supervision for flame sterilisation.
- Incubate at no more than 25 °C, tape without fully sealing to avoid anaerobic conditions, store plates upside down and never reopen them.
- Wear eye protection, disinfect spills as instructed and dispose of cultures through the school's sterilisation procedure.
Typical results and conclusion
A larger clear zone suggests stronger inhibition under the tested conditions. Compare repeats and the solvent control; zone size does not by itself show that a treatment is safe or effective in a patient.
Calculation: Compare mean zone diameter, or calculate zone area with area = πr² when the question asks for it.
Common exam mistake
Treating a large clear zone as proof that a treatment is safe or effective inside a person.
Revise it in the Cell Biology notes →Full Biology practicals guide →
Biology RP 3Combined + SeparateTrilogy RP 2
Investigate osmosis in plant tissue
Aim: Measure how a range of salt or sugar concentrations changes the mass of equal plant-tissue pieces.
Variables
- Independent
- concentration of the salt or sugar solution
- Dependent
- percentage change in mass of the plant tissue
- Control
- plant species and source tissue
- initial cylinder length and diameter
- solution volume
- immersion time and temperature
- blotting method before each weighing
Method
- Prepare labelled tubes containing equal volumes of a range of salt or sugar concentrations, including distilled water.
- Cut plant-tissue cylinders to equal length and diameter, remove any skin, blot them consistently and record each initial mass.
- Place one cylinder in each solution for the same time at the same temperature.
- Remove each cylinder, blot it in the same way and record its final mass.
- Calculate percentage change in mass for every concentration.
- Repeat each concentration, calculate a mean and plot mean percentage change against concentration.
Risks and safety
- Use a tile and cut away from fingers when using a cork borer or scalpel; follow teacher supervision.
- Wear eye protection if the chosen solutions require it and wipe up spills promptly.
Typical results and conclusion
Positive percentage change means net water entry; negative change means net water loss. The graph's zero-change concentration estimates where there was no net movement of water.
Calculation: Percentage change in mass = (final mass − initial mass) ÷ initial mass × 100.
Common exam mistake
Comparing raw mass changes between unequal pieces instead of percentage change, or forgetting to blot before weighing.
Revise it in the Cell Biology notes →Full Biology practicals guide →
Biology RP 4Combined + SeparateTrilogy RP 3
Use qualitative tests to identify food groups
Aim: Use visible results from standard reagents to test prepared food samples for reducing sugars, starch, protein and lipids.
Variables
- Independent
- food sample being compared while one food-group test is kept fixed
- Dependent
- the observed colour or layer produced by that fixed test
- Control
- sample preparation and volume
- identity and volume of the reagent or reagents for the chosen test
- heating time and water-bath temperature for Benedict's test
- clean apparatus for each sample
Method
- Prepare a food solution and divide it into separate, labelled samples.
- Add iodine solution to one sample; a blue-black result indicates starch.
- Add Benedict's solution to another sample and warm it in a water bath; a change away from blue towards green, yellow, orange or brick-red indicates reducing sugar.
- Add Biuret reagent to a fresh sample; a lilac or purple result indicates protein.
- Shake a fresh sample with ethanol, then add distilled water; a cloudy white emulsion indicates lipid.
- Compare each observation with a negative control and repeat uncertain results.
Risks and safety
- Wear eye protection and follow the reagent hazard guidance supplied by the school.
- Ethanol is highly flammable; keep it stoppered and well away from naked flames, hot equipment and other ignition sources.
- Biuret reagent contains corrosive sodium hydroxide and poisonous copper sulfate; avoid contact and rinse skin spills immediately.
- Heat Benedict's test indirectly in a water bath; use a holder for hot tubes and point them away from people.
Typical results and conclusion
A qualitative test identifies presence rather than an exact concentration. Record the starting and final appearance and compare with a positive control. A negative Benedict's result does not rule out a non-reducing sugar such as sucrose.
Calculation: This is qualitative evidence: record the starting appearance and the final positive or negative result rather than inventing a concentration.
Common exam mistake
Mixing up the reagent-result pairs or heating every test as though it were Benedict’s test.
Revise it in the Organisation notes →Full Biology practicals guide →
Biology RP 5Combined + SeparateTrilogy RP 4
Investigate the effect of pH on amylase
Aim: Measure how pH affects the time taken for amylase to digest starch while keeping temperature constant.
Variables
- Independent
- pH of the reaction mixture
- Dependent
- time until starch is no longer detected, or rate calculated from that time
- Control
- temperature
- amylase concentration and volume
- starch concentration and volume
- 30-second sampling interval
- total reaction volume
Method
- Place separate drops of iodine solution into the wells of a spotting tile.
- Bring measured starch, amylase and buffer solutions to the chosen water-bath temperature.
- Mix the starch and amylase with a buffer of known pH, start timing at once, and keep the mixture in the water bath throughout sampling.
- Every 30 seconds, transfer a drop of the reaction mixture to a fresh iodine drop.
- Record the first time at which the iodine remains orange-brown, showing that starch is no longer detected.
- Repeat at other pH values and repeat each condition before comparing mean times or calculated rates.
Risks and safety
- Wear eye protection and avoid skin contact with iodine solution.
- Use a thermostatically controlled water bath at a safe temperature.
- Use clean transfer equipment and wipe up spills promptly.
Typical results and conclusion
A shorter disappearance time indicates a faster reaction. Plot rate (1/time, s⁻¹) against pH to identify the optimum under the tested conditions; 1000/time may be plotted if the axis is labelled 'rate / 10⁻³ s⁻¹'.
Calculation: Rate is proportional to 1 ÷ time. If you use a scaled reciprocal such as 1000 ÷ time, define it consistently.
Common exam mistake
Mixing the starch, amylase and buffer before each has reached the water-bath temperature, calling the shortest time the lowest rate, or changing the sampling interval between pH values.
Revise it in the Organisation notes →Full Biology practicals guide →
Biology RP 6Combined + SeparateTrilogy RP 5
Investigate light intensity and photosynthesis rate
Aim: Measure how changing light intensity affects the photosynthesis rate of an aquatic organism such as pondweed.
Variables
- Independent
- distance from the lamp or calculated light intensity
- Dependent
- mean oxygen-bubble count or oxygen volume released per unit time
- Control
- temperature, using a heat shield or water bath
- carbon dioxide concentration and solution volume
- pondweed species, length and orientation
- measurement and equilibration times
- lamp and background-light conditions
Method
- Place a freshly cut piece of pondweed in sodium hydrogencarbonate solution with its cut end pointing upwards.
- Position a lamp at a measured distance from the pondweed and allow the apparatus to equilibrate.
- For a fixed time, count oxygen bubbles or collect and measure the volume of oxygen released.
- Repeat the measurement at the same distance and calculate a mean rate in bubbles/min or cm³/min.
- Move the lamp to several measured distances, allowing equilibration and repeating the timed measurement each time.
- Keep temperature, carbon dioxide supply, pondweed species and length, solution volume and measurement time constant.
- Plot mean photosynthesis rate against distance or, at Higher Tier, calculated relative light intensity 1/d².
Risks and safety
- Keep water away from electrical connections and dry hands before adjusting the lamp or leads.
- A lamp can become hot; use a heat shield, avoid touching the bulb and allow it to cool before moving it.
- Wear eye protection if instructed and avoid splashing the solution.
Typical results and conclusion
Calculate a mean rate for each distance and identify anomalies. Gas volume is more valid than bubble count because bubble sizes vary. Higher Tier: use 1/d² to represent relative intensity.
Calculation: Calculate oxygen produced per unit time. Higher Tier may use relative light intensity = 1 ÷ distance².
Common exam mistake
Assuming bubble count is perfectly proportional to oxygen volume, or letting the lamp change the temperature too.
Revise it in the Bioenergetics notes →Full Biology practicals guide →
Biology RP 7Combined + SeparateTrilogy RP 6
Investigate how a factor affects human reaction time
Aim: Use a ruler-drop test to investigate how the hand used affects one participant's reaction time.
Variables
- Independent
- hand used to catch the ruler
- Dependent
- catch distance in centimetres, used as an indicator of reaction time
- Control
- same ruler and release position
- same participant posture and catching technique
- same person releasing the ruler
- same number of repeats and rest interval
- no countdown or intentional warning before release
Method
- Seat the participant with one forearm supported and their thumb and finger open beside the ruler's zero mark.
- Hold the ruler vertically so zero is level with the top of the participant's thumb, without giving a countdown.
- Release the ruler; the participant catches it as quickly as possible. Record the catch distance in centimetres.
- Repeat at least five times with the same hand, allowing short rests, then calculate a mean.
- Repeat the same procedure with the other hand and compare the mean catch distances.
- Repeat with more participants if making a group-level claim; present anonymised results and identify anomalies.
Risks and safety
- Keep the floor and movement area clear and perform the test while seated.
- Obtain the participant's agreement, stop if they report discomfort and do not collect names or medical information.
- Use a light plastic or wooden ruler with smooth, undamaged edges.
Typical results and conclusion
Calculate a mean catch distance for each hand after checking anomalies. A shorter mean indicates a faster reaction. Conclusions apply to the tested participants and conditions, not automatically to everyone.
Calculation: Calculate a mean for each condition after identifying anomalies. A shorter ruler-drop distance indicates a faster response; if a conversion table is given, convert each distance to a reaction time in seconds.
Common exam mistake
Giving a countdown, changing the release position or generalising from one participant to everybody.
Revise it in the Homeostasis and Response notes →Full Biology practicals guide →
Biology RP 8Separate Biology only
Investigate how light or gravity affects the growth of seedlings
Aim: Investigate how one-sided light affects the direction of growth in newly germinated seedlings.
Variables
- Independent
- direction from which light reaches the seedlings
- Dependent
- change in shoot angle and shoot length over the fixed growth period
- Control
- plant species and seedling age
- water volume and growing medium
- temperature and exposure time
- light intensity and distance from the lamp
- initial shoot size as closely as practical
Method
- Choose similar newly germinated seedlings and measure each shoot's initial length and angle.
- Make a careful, labelled biological drawing of each starting seedling at a consistent scale.
- Place one group in a light-proof box with a side opening so light arrives from one direction.
- Place a control group in otherwise identical conditions with light arriving evenly from above.
- Keep water, temperature, species, seedling age, growing medium, time and light intensity as similar as possible.
- After a fixed period, measure the shoot length and angle of growth for every seedling.
- Make a second careful, labelled biological drawing at the same scale to show the growth response.
- Calculate group means and compare both growth amount and bending direction.
Risks and safety
- Wash hands after handling seeds, seedlings and growing medium; do not eat laboratory plant material.
- Use a cool LED light and keep water away from electrical equipment.
- Handle scissors or craft tools only under teacher supervision when preparing boxes.
Typical results and conclusion
Compare mean angle changes, variation and sample sizes. Bending towards one-sided light supports positive phototropism; length data help separate directional bending from general growth differences.
Calculation: Compare mean change in shoot angle and mean growth. Photographs taken from the same position can make angle measurements more reliable.
Common exam mistake
Changing light direction and light intensity together, then pretending only direction caused the result.
Revise it in the Homeostasis and Response notes →Full Biology practicals guide →
Biology RP 9Combined + SeparateTrilogy RP 7
Investigate population size and species distribution in a habitat
Aim: Use quadrats to estimate a common species population and a transect to investigate how an environmental factor affects distribution.
Variables
- Independent
- position along the transect or the measured environmental factor associated with position
- Dependent
- abundance, frequency or percentage cover of the target species
- Control
- quadrat dimensions and boundary-count rule
- distance between transect samples
- species-identification method
- time of day and weather where practical
- instrument and measurement technique
- total sampling effort at each location
Method
- Define the habitat boundary, identify the target species reliably and measure or obtain the total area.
- Generate random coordinate pairs, locate each position with tape measures and place a quadrat of known area.
- Count the target species using the same rule for organisms touching the boundary; repeat at many random positions.
- Calculate mean abundance or density and scale it to the habitat area to estimate population size.
- For distribution, lay a tape across an environmental gradient and place quadrats at regular distances to form a belt transect.
- At each distance, record target-species abundance and measure the relevant abiotic factor with suitable equipment.
- Repeat with parallel transects where practical, calculate means and plot abundance and the factor against distance.
- Look for a pattern, quote data and discuss other variables before suggesting a causal explanation.
Risks and safety
- Complete the school's fieldwork risk assessment; wear suitable footwear and clothing and avoid steep, unstable, roadside or deep-water sites.
- Do not touch unknown plants, fungi, animals, waste or sharp objects; cover cuts and wash hands after fieldwork.
- Keep the group together, follow site boundaries and weather guidance, and carry equipment so tapes and quadrats do not become trip hazards.
- Minimise disturbance: replace moved objects, avoid trampling outside the sample and release any organism promptly if observation is permitted.
Typical results and conclusion
Calculate means and an estimated population with units. Plot suitable graphs and describe correlation, but use biological reasoning and control of other factors before claiming cause.
Calculation: Estimated population = mean count per quadrat × number of quadrat-sized areas in the habitat.
Common exam mistake
Placing quadrats where the species is easiest to see, then calling the sample random.
Revise it in the Ecology notes →Full Biology practicals guide →
Biology RP 10Separate Biology only
Separate Biology: investigate temperature and fresh-milk decay
Aim: Investigate how temperature affects the rate of decay of fresh milk by timing a fixed pH change.
Variables
- Independent
- temperature of the milk and lipase mixture in the water bath
- Dependent
- time to a fixed pH endpoint and calculated relative rate, 1 / time
- Control
- milk volume and starting batch
- lipase volume and concentration
- sodium carbonate volume and concentration
- indicator identity and volume
- starting pH and chosen endpoint
- equilibration time and mixing method
Method
- Label tubes for several temperatures; add equal volumes of fresh milk and sodium carbonate for the same alkaline start, then the same amount of Cresol red, to each.
- Prepare equal portions of the same lipase solution, which models decay by breaking milk fat into fatty acids that lower pH.
- Place each milk mixture and lipase portion in its water bath until both reach the target temperature; verify with a thermometer.
- Add the lipase to the milk, mix in the same way and start the timer immediately.
- Stop timing at one defined indicator-colour or pH endpoint and record the time in seconds.
- Repeat at each temperature, calculate a mean endpoint time and calculate relative rate = 1 / mean time.
- Plot mean relative rate against temperature and describe the pattern within the measured range.
Risks and safety
- Wear eye protection. Lipase, sodium carbonate and indicator can irritate skin or eyes; use prepared solutions, avoid aerosols and rinse splashes as instructed.
- Use thermostatically controlled water baths, heatproof mats and test-tube racks; take care with hot water and glassware.
- Use fresh pasteurised milk; check school allergy information, avoid skin contact, never taste materials, clean spills and follow the disposal procedure.
- Carry out the practical under trained school supervision and follow the reagent-specific risk assessment.
Typical results and conclusion
For each temperature, calculate a mean time and its reciprocal in s⁻¹. A larger relative rate means faster pH change; identify anomalies and do not extrapolate beyond the data.
Calculation: Relative rate = 1 ÷ mean time to the fixed pH endpoint, in s⁻¹. Calculate the mean time for each temperature before comparing rates.
Common exam mistake
Adding the lipase before the milk tube and the lipase tube have both reached the water-bath temperature, or comparing different colour or pH endpoints.
Revise it in the Ecology notes →Full Biology practicals guide →